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MEL-1 human ES cells labeled with the TG343 antibody, Cat. No. MAB4346 (upper, green) and overlayed with DAPI (lower, blue). Only pluripotent human E ...
TG343 Antibody, clone TG343
Description:
TG343 Antibody, clone TG343
Background Information:
Human embryonic stem cells (hESCs) have been traditionally characterised using a panel of antibodies to cell surface markers, such as SSEA-3, TRA-1-60 and TRA-1-81; however, none of these traditional markers are entirely specific to hESC cells (Laslett et al., 2003). The novel TG343 antibody, which has been shown to be a reliable cell surface marker for the detection and characterization of pluripotent human embryonic stem cells (Adewumi et al., 2007), reacts with a distinct epitope on the protein core of a high molecular weight pericellular matrix proteoglycan (Cooper et al., 2002). When this antibody is used in conjunction with the TG30 antibody, TG343 has been successfully shown through flow cytometric assays to be a robust and rapid tool for the purification and selection of live Oct-4-positive human embryonic stem cells from a mixed population of differentiated cell types.
Clone:
TG343
Specificity:
This antibody is specific for a distinct epitope on a novel 200 kDa pericellular keratan sulphate proteoglycan antigen expressed on the surface of pluripotent human embryonic stem cells.
Key Applications:
- Immunocytochemistry
- Flow Cytometry
- Immunofluorescence
- Western Blotting
Species Reactivity:
Human
Species Reactivity Note:
Reacts with human. Reactivity to other species has not yet been determined.
Immunogen:
Crude fraction of a lectin-bound glycoprotein from GCT 27 C4 conditioned medium
Molecular Weight:
200 kDa
Presentation:
Purified mouse monoclonal IgM in buffer containing PBS with 50% glycerol.
Storage Conditions:
Maintain frozen at -20°C in undiluted aliquots for up to 12 months fromd date of receipt.
Antibody Category:
Stem Cell Research
Antibody Sub-Category:
Pluripotent & Early Differentiation
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Concentration:
1 mg/ml
Trade Name:
Chemicon (Millipore)
Format:
Purified
Antibody Market:
Stem Cells
Antibody Type:
Monoclonal Antibody
Host:
Mouse