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Western Blot Analysis:
HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-hnRNP K (1:2000 dilution).
Anti-hnRNP K, clone F45P9C7
Description:
Anti-hnRNP K, clone F45P9C7
Background Information:
hnRNP K (Heterogeneous nuclear ribonucleoprotein K) is a pre-mRNA binding protein. It is a component of hnRNP complexes that act as substrates for pre-mRNA processing prior to translation in the cytoplasm. hnRNP K is ubiquitously expressed in both the cytoplasm and the nucleus. hnRNP K acts as transcriptional regulator and functions in nucleocytoplasmic shuttling. hnRNP K is modified by phosphorylation (PKC δ, c-Src), methylation, and acetylation, and its expression and function are increased by epidermal growth factor (EGF).
Alternate Names:
- Heterogeneous nuclear ribonucleoprotein K
- HNRPK
- TUNP
- CSBP
- FLJ41122
Clone:
clone F45P9C7
Specificity:
Antibody strongly detects a single band at ~62 kDa, corresponding to hnRNP K
Key Applications:
Western Blotting
Application Notes:
Western Blot Analysis: A 1:2000 dilution of this lot detected hnRNP K in HeLa cell lysate. Optimal working dilutions must be determined by the end user.
Species Reactivity:
Human
Species Reactivity Note:
Reacts with human.
Predicted to react with mouse, rat, chicken, and rabbit, due to sequence homology.
Predicted to react with mouse, rat, chicken, and rabbit, due to sequence homology.
Isotype:
IgG1κ
Immunogen:
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of human hnRNP K
Epitope:
C-Terminus
Molecular Weight:
62 kDa
Control:
HeLa cell lysate
Presentation:
Mouse ascites IgG1k in buffer containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Gene Symbol:
- HNRPK
- HNRNPK
Quality Assurance:
Routinely evaluated by western blot on HeLa cell lysate.
Antibody Category:
Nuclear Function
Antibody Sub-Category:
RNA Metabolism & Binding Proteins
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
The ribonucleoprotein (RNP) domain is one of the most common eukaryotic protein folds. RNP domain proteins direct the assembly of multiprotein complexes on primary transcripts, mature mRNAs, and stable ribonucleoprotein components of the RNA processing machinery.
Trade Name:
Upstate (Millipore)
Purification Method:
Ascites
Format:
Ascites
Protein Type:
pre-mRNA binding protein
Antibody Type:
Monoclonal Antibody
Protein or Enzyme Type:
ribonucleoprotein
Host:
Mouse